JUP ELISA Kit

Catalog No. ABIN7830476

This Colorimetric ELISA kit is designed for the quantitative measurement of Human JUP.

Quick Overview for JUP ELISA Kit (ABIN7830476)

Target: JUP (Junction Plakoglobin (JUP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details JUP ELISA Kit

Purpose: JUP (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of JUP expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for JUP

Target: JUP (Junction Plakoglobin (JUP))

Alternative Name: JUP

Background:

Synonyms: ARVD12|CTNNG|DP3|DPIII|PDGB|PKGB

Gene Description: junction plakoglobin

Gene ID: 3728

UniProt: P14923

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location