CD247 ELISA Kit

Catalog No. ABIN7830483

The Human CD247 ELISA Kit (ABIN7830483) is a Colorimetric ELISA Kit designed to quantify Human CD247.

Quick Overview for CD247 ELISA Kit (ABIN7830483)

Target: CD247 (CD247 Molecule (CD247))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details CD247 ELISA Kit

Purpose: CD247 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CD247 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for CD247

Target: CD247 (CD247 Molecule (CD247))

Alternative Name: CD247

Background:

Synonyms: CD3-ZETA|CD3H|CD3Q|CD3Z|T3Z|TCRZ

Gene Description: CD247 Molecule

Gene ID: 919

UniProt: P20963

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway