CD40 ELISA Kit

Catalog No. ABIN7830485

This Colorimetric ELISA kit is designed for the quantitative measurement of Human CD40.

Quick Overview for CD40 ELISA Kit (ABIN7830485)

Target: CD40

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details CD40 ELISA Kit

Purpose: CD40 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CD40 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for CD40

Target: CD40

Alternative Name: CD40

Background:

Synonyms: Bp50|CDW40|MGC9013|TNFRSF5|p50

Gene Description: CD40 Molecule, TNF receptor superfamily member 5

Gene ID: 958

UniProt: P25942

Pathways: NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints