CD5 ELISA Kit
Quick Overview for CD5 ELISA Kit (ABIN7830486)
Target
See all CD5 ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
-
-
Purpose
- CD5 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CD5 expression in cultured cells.
-
Analytical Method
- Qualitative
-
Cross-Reactivity
- Mouse, Rat
-
-
CD5 (CD5) ELISA Kit
CD5 Reactivity: Human Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
CD5 (CD5) ELISA KitCD5 Reactivity: Human AA 25-371 Colorimetric Sandwich ELISA 93.7-6000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
-
-
Application Notes
- Optimal working dilution should be determined by the investigator.
-
Plate
- Uncoated
-
Protocol
-
- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
-
Restrictions
- For Research Use only
-
-
-
Storage
- 4 °C
-
Storage Comment
- Store the kit at 4°C.
-
-
- CD5
-
Alternative Name
- CD5
-
Background
-
Synonyms: LEU1|T1
Gene Description: CD5 Molecule
-
Gene ID
- 921
-
UniProt
- P06127
Target See all CD5 ELISA Kits
-
