C5AR1 ELISA Kit

Catalog No. ABIN7830489

The Human C5AR1 ELISA Kit (ABIN7830489) is a Colorimetric ELISA Kit designed to quantify Human C5AR1.

Quick Overview for C5AR1 ELISA Kit (ABIN7830489)

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details C5AR1 ELISA Kit

Purpose: C5AR1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of C5AR1 expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for C5AR1

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Alternative Name: C5AR1

Background:

Synonyms: C5A|C5AR|C5R1|CD88

Gene Description: complement component 5a receptor 1

Gene ID: 728

UniProt: P21730

Pathways: Complement System