Claudin 2 ELISA Kit
Quick Overview for Claudin 2 ELISA Kit (ABIN7830504)
Target
See all Claudin 2 (CLDN2) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- CLDN2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CLDN2 expression in cultured cells.
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Analytical Method
- Qualitative
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Cross-Reactivity
- Mouse
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Claudin 2 (CLDN2) ELISA Kit
CLDN2 Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Claudin 2 (CLDN2) ELISA KitCLDN2 Reactivity: Human Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Claudin 2 (CLDN2) ELISA KitCLDN2 Reactivity: Pig Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Claudin 2 (CLDN2) ELISA KitCLDN2 Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Uncoated
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Protocol
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- Store the kit at 4°C.
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- Claudin 2 (CLDN2)
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Alternative Name
- CLDN2
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Background
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Synonyms: -
Gene Description: claudin 2
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Gene ID
- 9075
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UniProt
- P57739
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Pathways
- Hepatitis C
Target See all Claudin 2 (CLDN2) ELISA Kits
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