CNOT2 ELISA Kit

Catalog No. ABIN7830510

The Human CNOT2 ELISA Kit (ABIN7830510) is a Colorimetric ELISA Kit designed to quantify Human CNOT2.

Quick Overview for CNOT2 ELISA Kit (ABIN7830510)

Target: CNOT2 (CCR4-NOT Transcription Complex, Subunit 2 (CNOT2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details CNOT2 ELISA Kit

Purpose: CNOT2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CNOT2 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for CNOT2

Target: CNOT2 (CCR4-NOT Transcription Complex, Subunit 2 (CNOT2))

Alternative Name: CNOT2

Background:

Synonyms: CDC36|HSPC131|NOT2|NOT2H

Gene Description: CCR4-NOT transcription complex, subunit 2

Gene ID: 4848

UniProt: Q9NZN8

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance