Cofilin 2 ELISA Kit
Quick Overview for Cofilin 2 ELISA Kit (ABIN7830512)
Target
See all Cofilin 2 (CFL2) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- CFL1/CFL2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CFL1/CFL2 expression in cultured cells.
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Analytical Method
- Qualitative
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Cross-Reactivity
- Mouse, Rat
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Cofilin 2 (CFL2) ELISA Kit
CFL2 Reactivity: Human Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Cofilin 2 (CFL2) ELISA KitCFL2 Reactivity: Mouse Colorimetric Sandwich ELISA 25 pg/mL - 1600 pg/mL Plasma, Serum, Tissue Homogenate
Cofilin 2 (CFL2) ELISA KitCFL2 Reactivity: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Uncoated
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Protocol
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- Store the kit at 4°C.
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- Cofilin 2 (CFL2)
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Alternative Name
- CFL2
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Background
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Synonyms: NEM7
Gene Description: cofilin 2 (muscle)
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Gene ID
- 1073
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UniProt
- P23528
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Pathways
- Caspase Cascade in Apoptosis
Target See all Cofilin 2 (CFL2) ELISA Kits
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