PLA2G4A ELISA Kit

Catalog No. ABIN7830523

Human PLA2G4A ELISA Kit, Colorimetric assay for quantification of Human PLA2G4A.

Quick Overview for PLA2G4A ELISA Kit (ABIN7830523)

Target: PLA2G4A (Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details PLA2G4A ELISA Kit

Purpose: PLA2G4A (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PLA2G4A expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for PLA2G4A

Target: PLA2G4A (Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))

Alternative Name: PLA2G4A

Background:

Synonyms: MGC126350|PLA2G4|cPLA2-alpha

Gene Description: phospholipase A2, group IVA (cytosolic, calcium-dependent)

Gene ID: 5321

UniProt: P47712

Pathways: Inositol Metabolic Process, G-protein mediated Events, VEGF Signaling