Dematin ELISA Kit

Catalog No. ABIN7830555

The Human Dematin ELISA Kit (ABIN7830555) is a Colorimetric ELISA Kit designed to quantify Human Dematin.

Quick Overview for Dematin ELISA Kit (ABIN7830555)

Target: Dematin (EPB49) (erythrocyte Membrane Protein Band 4.9 (Dematin) (EPB49))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details Dematin ELISA Kit

Purpose: EPB49 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of EPB49 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for Dematin

Target: Dematin (EPB49) (erythrocyte Membrane Protein Band 4.9 (Dematin) (EPB49))

Alternative Name: EPB49

Background:

Synonyms: DMT|FLJ78462|FLJ98848

Gene Description: erythrocyte membrane protein band 4.9 (dematin)

Gene ID: 2039

UniProt: Q08495

Pathways: Regulation of Actin Filament Polymerization