PARK7/DJ1 ELISA Kit

Catalog No. ABIN7830557

This Colorimetric ELISA kit is designed for the quantitative measurement of Human PARK7/DJ1.

Quick Overview for PARK7/DJ1 ELISA Kit (ABIN7830557)

Target: PARK7/DJ1 (PARK7) (Parkinson Protein 7 (PARK7))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details PARK7/DJ1 ELISA Kit

Purpose: PARK7 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PARK7 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for PARK7/DJ1

Target: PARK7/DJ1 (PARK7) (Parkinson Protein 7 (PARK7))

Alternative Name: PARK7

Background:

Synonyms: DJ-1|DJ1|FLJ27376|FLJ34360|FLJ92274

Gene Description: Parkinson disease (autosomal recessive, early onset) 7

Gene ID: 11315

UniProt: Q99497

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Proton Transport