CGB ELISA Kit

Catalog No. ABIN7830562

Human CGB ELISA Kit, Colorimetric assay for quantification of Human CGB.

Quick Overview for CGB ELISA Kit (ABIN7830562)

Target: CGB (Chorionic Gonadotropin, beta Polypeptide (CGB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details CGB ELISA Kit

Purpose: CGB (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CGB expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for CGB

Target: CGB (Chorionic Gonadotropin, beta Polypeptide (CGB))

Alternative Name: CGB

Background:

Synonyms: CGB3|hCGB

Gene Description: chorionic gonadotropin, beta polypeptide

Gene ID: 1082

UniProt: P01233