HDAC5 ELISA Kit

Catalog No. ABIN7830568

Human HDAC5 ELISA Kit, Colorimetric assay for quantification of Human HDAC5.

Quick Overview for HDAC5 ELISA Kit (ABIN7830568)

Target: HDAC5 (Histone Deacetylase 5 (HDAC5))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details HDAC5 ELISA Kit

Purpose: HDAC5/HDAC9 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of HDAC5/HDAC9 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for HDAC5

Target: HDAC5 (Histone Deacetylase 5 (HDAC5))

Alternative Name: HDAC5

Background:

Synonyms: FLJ90614|HD5|NY-CO-9

Gene Description: histone deacetylase 5

Gene ID: 10014

UniProt: Q9UQL6

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Monocarboxylic Acid Catabolic Process