HGS ELISA Kit

Catalog No. ABIN7830575

This Colorimetric ELISA kit is designed for the quantitative measurement of Human HGS.

Quick Overview for HGS ELISA Kit (ABIN7830575)

Target: HGS (Hepatocyte Growth Factor-Regulated tyrosine Kinase Substrate (HGS))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details HGS ELISA Kit

Purpose: HGS (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of HGS expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for HGS

Target: HGS (Hepatocyte Growth Factor-Regulated tyrosine Kinase Substrate (HGS))

Alternative Name: HGS

Background:

Synonyms: HRS|ZFYVE8

Gene Description: hepatocyte growth factor-regulated tyrosine kinase substrate

Gene ID: 9146

UniProt: O14964

Pathways: EGFR Signaling Pathway, CXCR4-mediated Signaling Events, Synaptic Vesicle Exocytosis, EGFR Downregulation