HIST1H3A ELISA Kit

Catalog No. ABIN7830579

The Human HIST1H3A ELISA Kit (ABIN7830579) is a Colorimetric ELISA Kit designed to quantify Human HIST1H3A.

Quick Overview for HIST1H3A ELISA Kit (ABIN7830579)

Target: HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details HIST1H3A ELISA Kit

Purpose: Histone H3.1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of Histone H3 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for HIST1H3A

Target: HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Alternative Name: HIST1H3A

Background:

Synonyms: H3/A|H3FA

Gene Description: histone cluster 1, H3a

Gene ID: 8350

UniProt: P68431