HCLS1 ELISA Kit

Catalog No. ABIN7830586

Human HCLS1 ELISA Kit, Colorimetric assay for quantification of Human HCLS1.

Quick Overview for HCLS1 ELISA Kit (ABIN7830586)

Target: HCLS1 (Hematopoietic Cell-Specific Lyn Substrate 1 (HCLS1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details HCLS1 ELISA Kit

Purpose: HCLS1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of HCLS1 expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for HCLS1

Target: HCLS1 (Hematopoietic Cell-Specific Lyn Substrate 1 (HCLS1))

Alternative Name: HCLS1

Background:

Synonyms: CTTNL|HS1

Gene Description: hematopoietic cell-specific Lyn substrate 1

Gene ID: 3059

UniProt: P14317

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Maintenance of Protein Location