IL-10RA ELISA Kit
Quick Overview for IL-10RA ELISA Kit (ABIN7830601)
Target
See all IL-10RA (IL10RA) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- IL10RA (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of IL10RA expression in cultured cells.
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Analytical Method
- Qualitative
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Interleukin 10 Receptor, alpha (IL10RA) ELISA Kit
IL10RA Reactivity: Human Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Tissue Homogenate
Interleukin 10 Receptor, alpha (IL10RA) ELISA KitIL10RA Reactivity: Mouse Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Tissue Homogenate
Interleukin 10 Receptor, alpha (IL10RA) ELISA KitIL10RA Reactivity: Human Colorimetric Sandwich ELISA 2.5-500 ng/mL Cell Culture Supernatant, Plasma, Serum
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Uncoated
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Protocol
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- Store the kit at 4°C.
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- IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))
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Alternative Name
- IL10RA
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Background
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Synonyms: CDW210A|HIL-10R|IL-10R1|IL10R
Gene Description: interleukin 10 receptor, alpha
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Gene ID
- 3587
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UniProt
- Q13651
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Pathways
- Growth Factor Binding
Target See all IL-10RA (IL10RA) ELISA Kits
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