UBR5 ELISA Kit

Catalog No. ABIN7830612

The Human UBR5 ELISA Kit (ABIN7830612) is a Colorimetric ELISA Kit designed to quantify Human UBR5.

Quick Overview for UBR5 ELISA Kit (ABIN7830612)

Target: UBR5 (Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details UBR5 ELISA Kit

Purpose: UBR5 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of UBR5 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for UBR5

Target: UBR5 (Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5))

Alternative Name: UBR5

Background:

Synonyms: DD5|EDD|EDD1|FLJ11310|HYD|KIAA0896|MGC57263

Gene Description: ubiquitin protein ligase E3 component n-recognin 5

Gene ID: 51366

UniProt: O95071

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway