EEF2 ELISA Kit

Catalog No. ABIN7830613

Human EEF2 ELISA Kit, Colorimetric assay for quantification of Human EEF2.

Quick Overview for EEF2 ELISA Kit (ABIN7830613)

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details EEF2 ELISA Kit

Purpose: EEF2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of EEF2 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for EEF2

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Alternative Name: EEF2

Background:

Synonyms: EEF-2|EF2

Gene Description: eukaryotic translation elongation factor 2

Gene ID: 1938

UniProt: P13639

Pathways: AMPK Signaling