ESR2 ELISA Kit

Catalog No. ABIN7830637

Human ESR2 ELISA Kit, Colorimetric assay for quantification of Human ESR2.

Quick Overview for ESR2 ELISA Kit (ABIN7830637)

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details ESR2 ELISA Kit

Purpose: ESR2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of ESR2 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for ESR2

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Alternative Name: ESR2

Background:

Synonyms: ER-BETA|ESR-BETA|ESRB|ESTRB|Erb|NR3A2

Gene Description: estrogen receptor 2 (ER beta)

Gene ID: 2100

UniProt: Q92731

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling