Filamin A ELISA Kit
Quick Overview for Filamin A ELISA Kit (ABIN7830652)
Target
See all Filamin A (FLNA) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
-
-
Purpose
- FLNA (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of FLNA expression in cultured cells.
-
Analytical Method
- Qualitative
-
Cross-Reactivity
- Mouse, Rat
-
-
Filamin A, alpha (FLNA) ELISA Kit
FLNA Reactivity: Human Colorimetric Sandwich ELISA 18.75 pg/mL - 1200 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Filamin A, alpha (FLNA) ELISA KitFLNA Reactivity: Human Colorimetric Sandwich ELISA 1.88 ng/mL - 120 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
-
-
Application Notes
- Optimal working dilution should be determined by the investigator.
-
Plate
- Uncoated
-
Protocol
-
- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
-
Restrictions
- For Research Use only
-
-
-
Storage
- 4 °C
-
Storage Comment
- Store the kit at 4°C.
-
-
- Filamin A (FLNA) (Filamin A, alpha (FLNA))
-
Alternative Name
- FLNA
-
Background
-
Synonyms: ABP-280|ABPX|DKFZp434P031|FLN|FLN1|FMD|MNS|NHBP|OPD|OPD1|OPD2
Gene Description: filamin A, alpha (actin binding protein 280)
-
Gene ID
- 2316
-
UniProt
- P21333
-
Pathways
- TCR Signaling, Maintenance of Protein Location
Target See all Filamin A (FLNA) ELISA Kits
-
