FOXN2 ELISA Kit

Catalog No. ABIN7830658

Human FOXN2 ELISA Kit, Colorimetric assay for quantification of Human FOXN2.

Quick Overview for FOXN2 ELISA Kit (ABIN7830658)

Target: FOXN2 (Forkhead Box N2 (FOXN2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details FOXN2 ELISA Kit

Purpose: FOXN2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of FOXN2 expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for FOXN2

Target: FOXN2 (Forkhead Box N2 (FOXN2))

Alternative Name: FOXN2

Background:

Synonyms: HTLF

Gene Description: forkhead box N2

Gene ID: 3344

UniProt: P32314