GANP ELISA Kit

Catalog No. ABIN7830670

Human GANP ELISA Kit, Colorimetric assay for quantification of Human GANP.

Quick Overview for GANP ELISA Kit (ABIN7830670)

Target: GANP (MCM3AP) (Minichromosome Maintenance Complex Component 3 Associated Protein (MCM3AP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details GANP ELISA Kit

Purpose: MCM3AP (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of MCM3AP expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for GANP

Target: GANP (MCM3AP) (Minichromosome Maintenance Complex Component 3 Associated Protein (MCM3AP))

Alternative Name: MCM3AP

Background:

Synonyms: FLJ44336|FLJ45306|GANP|KIAA0572|MAP80

Gene Description: minichromosome maintenance complex component 3 associated protein

Gene ID: 8888

UniProt: O60318

Pathways: Protein targeting to Nucleus