GRB10 ELISA Kit

Catalog No. ABIN7830686

This Colorimetric ELISA kit is designed for the quantitative measurement of Human GRB10.

Quick Overview for GRB10 ELISA Kit (ABIN7830686)

Target: GRB10 (Growth Factor Receptor-Bound Protein 10 (GRB10))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details GRB10 ELISA Kit

Purpose: GRB10 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of GRB10 expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for GRB10

Target: GRB10 (Growth Factor Receptor-Bound Protein 10 (GRB10))

Alternative Name: GRB10

Background:

Synonyms: GRB-IR|Grb-10|IRBP|KIAA0207|MEG1|RSS

Gene Description: growth factor receptor-bound protein 10

Gene ID: 2887

UniProt: Q13322

Pathways: Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2