NOS2 ELISA Kit
Quick Overview for NOS2 ELISA Kit (ABIN7830700)
Target
See all NOS2 ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- NOS2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of NOS2 expression in cultured cells.
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Analytical Method
- Qualitative
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Nitric Oxide Synthase 2, Inducible (NOS2) ELISA Kit
NOS2 Reactivity: Mouse Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 Reactivity: Rat Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 Reactivity: Rabbit Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 Reactivity: Rat Colorimetric Sandwich ELISA 0.78 IU/mL - 50 IU/mL Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 Reactivity: Human Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 Reactivity: Mouse Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitWide Range NOS2 Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitHigh Sensitivity NOS2 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Uncoated
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Protocol
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- Store the kit at 4°C.
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- NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))
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Alternative Name
- NOS2
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Background
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Synonyms: HEP-NOS|INOS|NOS|NOS2A
Gene Description: nitric oxide synthase 2, inducible
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Gene ID
- 4843
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UniProt
- P35228
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Pathways
- Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
Target See all NOS2 ELISA Kits
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