Cytokeratin 19 ELISA Kit
Quick Overview for Cytokeratin 19 ELISA Kit (ABIN7830722)
Target
See all Cytokeratin 19 (KRT19) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- KRT19 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of KRT19 expression in cultured cells.
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Analytical Method
- Qualitative
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Cross-Reactivity
- Mouse, Rat
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Keratin 19 (KRT19) ELISA Kit
KRT19 Reactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Keratin 19 (KRT19) ELISA KitKRT19 Reactivity: Mouse Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Uncoated
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Protocol
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- Store the kit at 4°C.
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- Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))
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Alternative Name
- KRT19
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Background
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Synonyms: CK19|K19|K1CS|MGC15366
Gene Description: keratin 19
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Gene ID
- 3880
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UniProt
- P08727
Target See all Cytokeratin 19 (KRT19) ELISA Kits
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