KCNJ11 ELISA Kit

Catalog No. ABIN7830728

This Colorimetric ELISA kit is designed for the quantitative measurement of Human KCNJ11.

Quick Overview for KCNJ11 ELISA Kit (ABIN7830728)

Target: KCNJ11 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 11 (KCNJ11))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details KCNJ11 ELISA Kit

Purpose: KCNJ11 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of KCNJ11 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for KCNJ11

Target: KCNJ11 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 11 (KCNJ11))

Alternative Name: KCNJ11

Background:

Synonyms: BIR|HHF2|IKATP|KIR6.2|MGC133230|PHHI|TNDM3

Gene Description: potassium inwardly-rectifying channel, subfamily J, member 11

Gene ID: 3767

UniProt: Q14654

Pathways: Negative Regulation of Hormone Secretion