MMP14 ELISA Kit

Catalog No. ABIN7830772

Human MMP14 ELISA Kit, Colorimetric assay for quantification of Human MMP14.

Quick Overview for MMP14 ELISA Kit (ABIN7830772)

Target: MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details MMP14 ELISA Kit

Purpose: MMP14 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of MMP14 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for MMP14

Target: MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))

Alternative Name: MMP14

Background:

Synonyms: MMP-X1|MT1-MMP|MTMMP1

Gene Description: matrix metallopeptidase 14 (membrane-inserted)

Gene ID: 4323

UniProt: P50281

Pathways: Autophagy