MMP23B ELISA Kit

Catalog No. ABIN7830777

The Human MMP23B ELISA Kit (ABIN7830777) is a Colorimetric ELISA Kit designed to quantify Human MMP23B.

Quick Overview for MMP23B ELISA Kit (ABIN7830777)

Target: MMP23B (Matrix Metallopeptidase 23B (MMP23B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details MMP23B ELISA Kit

Purpose: MMP23B (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of MMP23B expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for MMP23B

Target: MMP23B (Matrix Metallopeptidase 23B (MMP23B))

Alternative Name: MMP23B

Background:

Synonyms: MIFR|MIFR-1|MMP22

Gene Description: matrix metallopeptidase 23B

Gene ID: 8510

UniProt: O75900