MMP3 ELISA Kit
Quick Overview for MMP3 ELISA Kit (ABIN7830778)
Target
See all MMP3 ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
-
-
Purpose
- MMP3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of MMP3 expression in cultured cells.
-
Analytical Method
- Qualitative
-
Cross-Reactivity
- Mouse, Rat
-
-
-
-
Application Notes
- Optimal working dilution should be determined by the investigator.
-
Plate
- Uncoated
-
Protocol
-
- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
-
Restrictions
- For Research Use only
-
-
-
Storage
- 4 °C
-
Storage Comment
- Store the kit at 4°C.
-
-
- MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))
-
Alternative Name
- MMP3
-
Background
-
Synonyms: CHDS6|MGC126102|MGC126103|MGC126104|MMP-3|SL-1|STMY|STMY1|STR1
Gene Description: matrix metallopeptidase 3 (stromelysin 1, progelatinase)
-
Gene ID
- 4314
-
UniProt
- P08254
Target See all MMP3 ELISA Kits
-