CSTA ELISA Kit
Quick Overview for CSTA ELISA Kit (ABIN7830793)
Target
See all CSTA ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- CSTA (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CSTA expression in cultured cells.
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Analytical Method
- Qualitative
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Cystatin A (Stefin A) (CSTA) ELISA Kit
CSTA Reactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cystatin A (Stefin A) (CSTA) ELISA KitCSTA Reactivity: Mouse Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Saliva, Serum, Tissue Homogenate
Cystatin A (Stefin A) (CSTA) ELISA KitCSTA Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Uncoated
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Protocol
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- Store the kit at 4°C.
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- CSTA (Cystatin A (Stefin A) (CSTA))
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Alternative Name
- CSTA
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Background
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Synonyms: STF1|STFA
Gene Description: cystatin A (stefin A)
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Gene ID
- 1475
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Pathways
- Response to Water Deprivation
Target See all CSTA ELISA Kits
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