S1PR1 ELISA Kit

Catalog No. ABIN7830796

Human S1PR1 ELISA Kit, Colorimetric assay for quantification of Human S1PR1.

Quick Overview for S1PR1 ELISA Kit (ABIN7830796)

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details S1PR1 ELISA Kit

Purpose: S1PR1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of S1PR1 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for S1PR1

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Alternative Name: S1PR1

Background:

Synonyms: CHEDG1|D1S3362|ECGF1|EDG-1|EDG1|FLJ58121|S1P1

Gene Description: sphingosine-1-phosphate receptor 1

Gene ID: 1901

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2