PPP1R12A ELISA Kit

Catalog No. ABIN7830816

The Human PPP1R12A ELISA Kit (ABIN7830816) is a Colorimetric ELISA Kit designed to quantify Human PPP1R12A.

Quick Overview for PPP1R12A ELISA Kit (ABIN7830816)

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details PPP1R12A ELISA Kit

Purpose: PPP1R12A (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PPP1R12A expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for PPP1R12A

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Alternative Name: PPP1R12A

Background:

Synonyms: MBS|MGC133042|MYPT1

Gene Description: protein phosphatase 1, regulatory (inhibitor) subunit 12A

Gene ID: 4659

Pathways: M Phase