NPM1 ELISA Kit

Catalog No. ABIN7830825

The Human NPM1 ELISA Kit (ABIN7830825) is a Colorimetric ELISA Kit designed to quantify Human NPM1.

Quick Overview for NPM1 ELISA Kit (ABIN7830825)

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details NPM1 ELISA Kit

Purpose: NPM1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of NPM1 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for NPM1

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Alternative Name: NPM1

Background:

Synonyms: B23|MGC104254|NPM

Gene Description: nucleophosmin (nucleolar phosphoprotein B23, numatrin)

Gene ID: 4869

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly