PDGFRB ELISA Kit

Catalog No. ABIN7830837

The Human PDGFRB ELISA Kit (ABIN7830837) is a Colorimetric ELISA Kit designed to quantify Human PDGFRB.

Quick Overview for PDGFRB ELISA Kit (ABIN7830837)

Target: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details PDGFRB ELISA Kit

Purpose: PDGFRB (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PDGFRB expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for PDGFRB

Target: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Alternative Name: PDGFRB

Background:

Synonyms: CD140B|JTK12|PDGF-R-beta|PDGFR|PDGFR1

Gene Description: platelet-derived growth factor receptor, beta polypeptide

Gene ID: 5159

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling