PMP22 ELISA Kit
Quick Overview for PMP22 ELISA Kit (ABIN7830847)
Target
See all PMP22 ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- PMP22 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PMP22 expression in cultured cells.
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Analytical Method
- Qualitative
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Cross-Reactivity
- Mouse, Rat
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Peripheral Myelin Protein 22 (PMP22) ELISA Kit
PMP22 Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Peripheral Myelin Protein 22 (PMP22) ELISA KitPMP22 Reactivity: Human Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
Peripheral Myelin Protein 22 (PMP22) ELISA KitPMP22 Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Peripheral Myelin Protein 22 (PMP22) ELISA KitPMP22 Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Uncoated
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Protocol
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- Store the kit at 4°C.
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- PMP22 (Peripheral Myelin Protein 22 (PMP22))
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Alternative Name
- PMP22
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Background
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Synonyms: CMT1A|CMT1E|DSS|GAS-3|HMSNIA|HNPP|MGC20769|Sp110
Gene Description: peripheral myelin protein 22
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Gene ID
- 5376
Target See all PMP22 ELISA Kits
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