PKC alpha ELISA Kit
Quick Overview for PKC alpha ELISA Kit (ABIN7830853)
Target
See all PKC alpha (PKCa) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- PRKCA (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PRKCA expression in cultured cells.
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Analytical Method
- Qualitative
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Cross-Reactivity
- Mouse, Rat
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Protein Kinase C, alpha (PKCa) ELISA Kit
PKCa Reactivity: Mouse Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reactivity: Rat Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reactivity: Mouse Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reactivity: Mouse Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Uncoated
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Protocol
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- Store the kit at 4°C.
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- PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
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Alternative Name
- PRKCA
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Background
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Synonyms: AAG6|MGC129900|MGC129901|PKC-alpha|PKCA|PRKACA
Gene Description: protein kinase C, alpha
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Gene ID
- 5578
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Pathways
- WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
Target See all PKC alpha (PKCa) ELISA Kits
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