Retinoic Acid Receptor beta ELISA Kit

Catalog No. ABIN7830873

The Human Retinoic Acid Receptor beta ELISA Kit (ABIN7830873) is a Colorimetric ELISA Kit designed to quantify Human Retinoic Acid Receptor beta.

Quick Overview for Retinoic Acid Receptor beta ELISA Kit (ABIN7830873)

Target: Retinoic Acid Receptor beta (RARB) (Retinoic Acid Receptor, beta (RARB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details Retinoic Acid Receptor beta ELISA Kit

Purpose: RARB (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of RARB expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for Retinoic Acid Receptor beta

Target: Retinoic Acid Receptor beta (RARB) (Retinoic Acid Receptor, beta (RARB))

Alternative Name: RARB

Background:

Synonyms: HAP|NR1B2|RRB2

Gene Description: retinoic acid receptor, beta

Gene ID: 5915

Pathways: Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway