SYK ELISA Kit

Catalog No. ABIN7830906

The Human SYK ELISA Kit (ABIN7830906) is a Colorimetric ELISA Kit designed to quantify Human SYK.

Quick Overview for SYK ELISA Kit (ABIN7830906)

Target: SYK (Spleen tyrosine Kinase (SYK))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details SYK ELISA Kit

Purpose: SYK (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of SYK expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for SYK

Target: SYK (Spleen tyrosine Kinase (SYK))

Alternative Name: SYK

Background:

Synonyms: DKFZp313N1010|FLJ25043|FLJ37489

Gene Description: spleen tyrosine kinase

Gene ID: 6850

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling, BCR Signaling