TIMP2 ELISA Kit
Quick Overview for TIMP2 ELISA Kit (ABIN7830917)
Target
See all TIMP2 ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- TIMP2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of TIMP2 expression in cultured cells.
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Analytical Method
- Qualitative
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Cross-Reactivity
- Mouse, Rat
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Metalloproteinase Inhibitor 2 (TIMP2) ELISA Kit
TIMP2 Reactivity: Mouse Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Metalloproteinase Inhibitor 2 (TIMP2) ELISA KitTIMP2 Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Metalloproteinase Inhibitor 2 (TIMP2) ELISA KitTIMP2 Reactivity: Human Colorimetric Sandwich ELISA 0.94 ng/mL - 60 ng/mL Plasma, Serum, Tissue Homogenate
Metalloproteinase Inhibitor 2 (TIMP2) ELISA KitTIMP2 Reactivity: Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Metalloproteinase Inhibitor 2 (TIMP2) ELISA KitTIMP2 Reactivity: Horse Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Plasma, Serum, Tissue Homogenate
Metalloproteinase Inhibitor 2 (TIMP2) ELISA KitWide Range TIMP2 Reactivity: Rat Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Metalloproteinase Inhibitor 2 (TIMP2) ELISA KitSmall Sample TIMP2 Reactivity: Mouse Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Metalloproteinase Inhibitor 2 (TIMP2) ELISA KitTIMP2 Reactivity: Chicken Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Metalloproteinase Inhibitor 2 (TIMP2) ELISA KitTIMP2 Reactivity: Guinea Pig Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Metalloproteinase Inhibitor 2 (TIMP2) ELISA KitTIMP2 Reactivity: Rabbit Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Uncoated
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Protocol
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- Store the kit at 4°C.
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- TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))
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Alternative Name
- TIMP2
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Background
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Synonyms: CSC-21K
Gene Description: TIMP metallopeptidase inhibitor 2
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Gene ID
- 7077
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Pathways
- cAMP Metabolic Process
Target See all TIMP2 ELISA Kits
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