Tumor Protein p73 ELISA Kit

Catalog No. ABIN7830923

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Tumor Protein p73.

Quick Overview for Tumor Protein p73 ELISA Kit (ABIN7830923)

Target: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details Tumor Protein p73 ELISA Kit

Purpose: TP73 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of TP73 expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for Tumor Protein p73

Target: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Alternative Name: TP73

Background:

Synonyms: P73

Gene Description: tumor protein p73

Gene ID: 7161

Pathways: Regulation of Cell Size, Positive Regulation of Response to DNA Damage Stimulus