UCHL1 ELISA Kit
Quick Overview for UCHL1 ELISA Kit (ABIN7830931)
Target
See all UCHL1 ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- UCHL1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of UCHL1 expression in cultured cells.
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Analytical Method
- Qualitative
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Cross-Reactivity
- Mouse, Rat
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Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) ELISA Kit
UCHL1 Reactivity: Human Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) ELISA KitUCHL1 Reactivity: Human, Rat, Mouse Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) ELISA KitUCHL1 Reactivity: Human Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) ELISA KitUCHL1 Reactivity: Rat Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) ELISA KitUCHL1 Reactivity: Mouse Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) ELISA KitUCHL1 Reactivity: Human Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate
Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) ELISA KitHigh Sensitivity UCHL1 Reactivity: Human C-Term Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) ELISA KitUCHL1 Reactivity: Human C-Term Colorimetric Sandwich ELISA 6.25-400 ng/mL Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate
Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) ELISA KitUCHL1 Reactivity: Rat C-Term Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Uncoated
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Protocol
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- Store the kit at 4°C.
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- UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))
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Alternative Name
- UCHL1
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Background
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Synonyms: PARK5|PGP9.5|Uch-L1
Gene Description: ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
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Gene ID
- 7345
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Pathways
- Feeding Behaviour
Target See all UCHL1 ELISA Kits
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