Vitamin D Receptor ELISA Kit

Catalog No. ABIN7830937

Human Vitamin D Receptor ELISA Kit, Colorimetric assay for quantification of Human Vitamin D Receptor.

Quick Overview for Vitamin D Receptor ELISA Kit (ABIN7830937)

Target: Vitamin D Receptor (VDR)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details Vitamin D Receptor ELISA Kit

Purpose: VDR (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of VDR expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for Vitamin D Receptor

Target: Vitamin D Receptor (VDR)

Alternative Name: VDR

Target Type: Chemical

Background:

Synonyms: NR1I1

Gene Description: vitamin D (1,25- dihydroxyvitamin D3) receptor

Gene ID: 7421

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway