Vimentin ELISA Kit
Quick Overview for Vimentin ELISA Kit (ABIN7830939)
Target
See all Vimentin (VIM) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- VIM (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of VIM expression in cultured cells.
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Analytical Method
- Qualitative
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Cross-Reactivity
- Mouse, Rat
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Vimentin (VIM) ELISA Kit
VIM Reactivity: Human Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vimentin (VIM) ELISA KitVIM Reactivity: Rat Colorimetric Sandwich ELISA 23.5 ng/mL - 1500 ng/mL Plasma, Serum, Tissue Homogenate
Vimentin (VIM) ELISA KitVIM Reactivity: Mouse Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Uncoated
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Protocol
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- Store the kit at 4°C.
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- Vimentin (VIM)
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Alternative Name
- VIM
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Background
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Synonyms: FLJ36605
Gene Description: vimentin
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Gene ID
- 7431
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Pathways
- Caspase Cascade in Apoptosis
Target See all Vimentin (VIM) ELISA Kits
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