RIPK2 ELISA Kit

Catalog No. ABIN7830951

The Human RIPK2 ELISA Kit (ABIN7830951) is a Colorimetric ELISA Kit designed to quantify Human RIPK2.

Quick Overview for RIPK2 ELISA Kit (ABIN7830951)

Target: RIPK2 (Receptor-Interacting Serine-threonine Kinase 2 (RIPK2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details RIPK2 ELISA Kit

Purpose: RIPK2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of RIPK2 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for RIPK2

Target: RIPK2 (Receptor-Interacting Serine-threonine Kinase 2 (RIPK2))

Alternative Name: RIPK2

Background:

Synonyms: CARD3|CARDIAK|CCK|GIG30|RICK|RIP2

Gene Description: receptor-interacting serine-threonine kinase 2

Gene ID: 8767

Pathways: TCR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades