PIKFYVE ELISA Kit

Catalog No. ABIN7830968

This Colorimetric ELISA kit is designed for the quantitative measurement of Human PIKFYVE.

Quick Overview for PIKFYVE ELISA Kit (ABIN7830968)

Target: PIKFYVE (phosphoinositide Kinase, FYVE Finger Containing (PIKFYVE))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Adherent Cell Culture, Cell Culture Cells, Cell Samples

Product Details PIKFYVE ELISA Kit

Purpose: PIP5K3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PIP5K3 expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for PIKFYVE

Target: PIKFYVE (phosphoinositide Kinase, FYVE Finger Containing (PIKFYVE))

Alternative Name: PIP5K3

Background:

Synonyms: CFD|FAB1|KIAA0981|MGC40423|PIKFYVE|PIP5K

Gene Description: phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III

Gene ID: 200576

Pathways: Inositol Metabolic Process