PKD2 ELISA Kit

Catalog No. ABIN7830970

Human PKD2 ELISA Kit, Colorimetric assay for quantification of Human PKD2.

Quick Overview for PKD2 ELISA Kit (ABIN7830970)

Target: PKD2 (Protein Kinase D2 (PKD2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Adherent Cell Culture, Cell Culture Cells, Cell Samples

Product Details PKD2 ELISA Kit

Purpose: PRKD2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PRKD2 expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for PKD2

Target: PKD2 (Protein Kinase D2 (PKD2))

Alternative Name: PRKD2

Background:

Synonyms: HSPC187|PKD2

Gene Description: protein kinase D2

Gene ID: 25865

Pathways: cAMP Metabolic Process, Maintenance of Protein Location, Negative Regulation of Transporter Activity