RPH3A ELISA Kit

Catalog No. ABIN7830971

This Colorimetric ELISA kit is designed for the quantitative measurement of Human RPH3A.

Quick Overview for RPH3A ELISA Kit (ABIN7830971)

Target: RPH3A (Rabphilin 3A (RPH3A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Adherent Cell Culture, Cell Culture Cells, Cell Samples

Product Details RPH3A ELISA Kit

Purpose: RPH3A (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of RPH3A expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for RPH3A

Target: RPH3A (Rabphilin 3A (RPH3A))

Alternative Name: RPH3A

Background:

Synonyms: KIAA0985

Gene Description: rabphilin 3A homolog (mouse)

Gene ID: 22895

Pathways: Synaptic Vesicle Exocytosis