PDE4C ELISA Kit

Catalog No. ABIN7830993

The Human PDE4C ELISA Kit (ABIN7830993) is a Colorimetric ELISA Kit designed to quantify Human PDE4C.

Quick Overview for PDE4C ELISA Kit (ABIN7830993)

Target: PDE4C (phosphodiesterase 4C, CAMP-Specific (PDE4C))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details PDE4C ELISA Kit

Purpose: PDE4D (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PDE4D expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 4°C for six months.

Target Details for PDE4C

Target: PDE4C (phosphodiesterase 4C, CAMP-Specific (PDE4C))

Alternative Name: PDE4C

Background:

Synonyms: DPDE1|MGC126222

Gene Description: phosphodiesterase 4C, cAMP-specific (phosphodiesterase E1 dunce homolog, Drosophila)

Gene ID: 5143

UniProt: Q08493

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process