ACER3 ELISA Kit

Catalog No. ABIN7830994

Human ACER3 ELISA Kit, Colorimetric assay for quantification of Human ACER3.

Quick Overview for ACER3 ELISA Kit (ABIN7830994)

Target: ACER3 (Alkaline Ceramidase 3 (ACER3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details ACER3 ELISA Kit

Purpose: ACER3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PHCA expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 4°C for six months.

Target Details for ACER3

Target: ACER3 (Alkaline Ceramidase 3 (ACER3))

Alternative Name: ACER3

Background:

Synonyms: APHC|FLJ11238|PHCA

Gene Description: alkaline ceramidase 3

Gene ID: 55331

UniProt: Q9NUN7